Real Coliform Test

 
1 Collect sample in clean container from effluent composite sampler.  
2 Bring sample back to lab.  
3 Pour sample into (2) whil-pal sterilized water sampling bags containing sodium thiosulfate to 100 ml. mark.  
4 Let sample sit until tablet in bag is dissolved. Check sample for 0.00 chlorine residual on DR 2000.  
5 Pour (1) sample in sterile Gelman microfunnel to 25 ml. mark and turn on vacuum pressure pump for filtering.  
6 Pour 25 ml. sterile phosphate solution into tunnel and filter.  
7 Turn off vacuum pump.